In summary, our results had shown that DARS/GDI2/P4HA2/TRUB1 can be used as a new biological marker for GBM, which is related to the occurrence and prognosis of GBM, how to rationally apply various genetic characteristics at specific stages of GBM for diagnose and prediction of prognosis. The gene discussed is TRUB1; the disease is glioblastoma.