Our data show that Chaihu-Shugan-San treats depression potentially via influence 110 DEPs (Gad2, Vamp2, Pde2a, etc.), and neurotransmitters release and transmission cycle (e.g., GABA, glutamate, serotonin, norepinephrine, dopamine, and acetylcholine). Here, VAMP2 is linked to depressive symptom measurement.