Our data show that Chaihu-Shugan-San treats depression potentially via influence 110 DEPs (Gad2, Vamp2, Pde2a, etc.), and neurotransmitters release and transmission cycle (e.g., GABA, glutamate, serotonin, norepinephrine, dopamine, and acetylcholine). The gene discussed is PDE2A; the disease is depressive disorder.