Our data show that Chaihu-Shugan-San treats depression potentially via influence 110 DEPs (Gad2, Vamp2, Pde2a, etc.), and neurotransmitters release and transmission cycle (e.g., GABA, glutamate, serotonin, norepinephrine, dopamine, and acetylcholine). Here, GAD2 is linked to major depressive disorder.