Notably, various activation patterns of Rac/Cdc42 were observed in colon cancer FFPE tissues, designated plasma membrane, cytoplasmic, mixed membrane and cytoplasm, and polarized patterns (Fig. 4b, Supplemental Table S2), suggesting functional variation of activated Rac and Cdc42 in human colon cancer tissues. The gene discussed is AKT1; the disease is malignant colon neoplasm.