These genes included CCL2, RELB, IL32, PLAT, PTGES, PHLDA1 and TNFRSF9. The relative fold-changes of the genes in the human neuroblastoma SH-SY5Y cells transfected with recombinant plasmid expressing 2BmCherry, namely pLVX-puro::FLAG-2B-mCherry, were calculated by the relative Ct values to those of the cells transfected with recombinant plasmid expressing mCherry, namely pLenti::mCherry, normalized with the Ct value of the respective gene amplified from the normal cells by 2−∆∆Ct method. The gene discussed is PHLDA1; the disease is neuroblastoma.