To understand how Bmal1-depleted astrocytes behave in the context of Aβ pathology, we examined astrocyte activation in the cortex of BMAL1 aKO mice crossed to the APP/PS1-21 model of Aβ-amyloidosis (Aldh1l1-CreERT2;Bmal1fl/fl;APP/PS1-21). Here, APP is linked to amyloidosis.