Therefore, to combine CD4 and CD8 neoantigens with ICI in a second tumor model, we selected CD4 and CD8 neoantigens expressed in the CT26 colon cancer cells37 and we generated C20, a vaccine expressing twenty CT26 neoantigens in the form of 28-mers (Supplementary Table 1). Here, CD8A is linked to malignant colon neoplasm.