POLR2A and infection: To determine whether transcriptional host shut off and tRNA upregulation may be linked phenomena, we performed ChIP-Seq for POLR2A on human fibroblasts after infection with ∆ICP0/4/22/27/47, ∆ICP4, ∆ICP27, ∆ICP22, and wildtype HSV-1 for 6 h (Supplementary Fig. 8).