We compared the expression levels of the above genes between the cancer tissues and adjacent normal tissues, and found that ANKZF1, STC2 and P4HA1 were significantly upregulated, while SUCLG2P2 and PCK1 were downregulated in both COAD and READ among the independent prognostic genes of COAD (Fig. 1). Here, ANKZF1 is linked to cancer.