We used TIMER 2.0 to analyze the immune cell infiltration of G34-DHGs with MUC16 mutation and G34-DHGs with wild-type MUC16. However, no connection between MUC16 mutation and immune cell infiltration was found; this may be because of the small number of cases and relatively low immune infiltration of G34 glioma. The gene discussed is MUC16; the disease is glioma.