For deeply understanding the influence of MEG3/miR-494-3p/OTUD4 as the regulatory axis on breast cancer cells, MEG3, miR-494-3p and OTUD4 expression in 4 groups (oe-NC  +  sh-NC group, oe-MEG3  +  sh-NC group oe-MEG3  +  sh-OTUD4-1 group and oe-MEG3  +  sh-OTUD4-2 group) was tested by qRT-PCR (Fig. 4A), and OTUD4 protein expression was measured by western blot (Fig. 4B). Here, OTUD4 is linked to breast cancer.