To confirm that CD69+CD103+ T cells that expand after RSV re-infection are localized within the tissue and not the vasculature, CD45+ cells in circulation were intravenously labeled 10 minutes before euthanasia with BUV395 conjugated anti-CD45 antibody (CD45 i.v.)and TRM cells were analyzed at days 0, 2 and 4 post re-infection (gating strategy S1C Fig). Here, CD69 is linked to infection.