In addition, the heatmap constructed from the flow cytometric dot plots exhibited a dysfunctional state of the tumor-infiltrating eEF-2K KO CAR-T cells: Compared to WT CD8+ CAR-T cells, the CD8+ CAR-T cells lacking eEF-2K produced significantly less inflammatory cytokines TNF-α, IFN-γ, and IL-4, and the prosurvival cytokine IL-1α, but an equivalent amount of IL-2 cytokine (Fig. 6C and fig. This evidence concerns the gene TNF and neoplasm.