Moreover, to further confirm whether the up-stream signaling pathway of IFIT2, namely, JAK/STAT was involved in the regulation of the cellular function upon PD-L1 knockdown expression, we selected the JAK inhibitor AG490 to treat the esophageal cancer cell lines and compare the cellular functions as well as EMT phenotypes between LV-NC and LV-shPD-L1 groups. This evidence concerns the gene CD274 and esophageal cancer.