To test whether PPM1D phosphatase activity is required for accelerated glioma formation in our models, we performed IUE of H3.3K27M and PdgfraD842V plasmids along with a mutant form of PPM1Dtr (PPM1Dtr-D314A), that inactivates the phosphatase activity15. The gene discussed is PPM1D; the disease is central nervous system cancer.