CD4 and neoplasm: To confirm whether PD-1–laIL-2 can increase the effector function of some subsets of TILs, we sorted CD4+, PD-1–CD8+, PD-1+TIM3–CD8+, and PD-1+TIM3+CD8+ T cells from the tumor and treated them with PD-1–laIL-2 in the presence of irradiated A20 tumor cells (Figure 7A).