In conclusion, our RNA-Seq data from VICs in which increased Ca2+ influx through CaV1.2 identified multiple differentially expressed genes and signaling pathways common in CAVD, show that the association between CACNA1C SNPs and increased Ca2+ influx through CaV1.2 with CAVD is, indeed, causal. This evidence concerns the gene CACNA1C and congenital bilateral aplasia of vas deferens from CFTR mutation.