To verify the functional effects of the NANOG/HDAC1 axis in diverse types of mouse or human cancer cells, we further selected 3 NANOG-upregulated cancer cells, B16F10, 526mel, and H1299, and 3 NANOGhi immunotherapeutic-refractory tumor models, MDA-MB-231 P3, CT26 P3, and YUMM2.1 P3, respectively (28). The gene discussed is HDAC1; the disease is cancer.