HSPA5 and acute myeloid leukemia: To demonstrate cell surface expression of GRP78, we performed flow cytometry of AML cell lines (KG1a, MOLM13, THP-1, MV4-11) using an antibody specific for the ER retention sequence (KDEL) at the C-terminus of GRP78 or a biotin-conjugated peptide (Biotin-Ahx-CTVALPGGYVRVC) that specifically binds GRP7823.