We quantified the protein blots by the ImageJ software and found that the expression of ST3GAL4, TRIM8, and STC2 genes in both MG63 cells and SJSA-1 cells was higher than that in the hFOB1.19 cells, which further demonstrated that the expression of these three genes in osteosarcoma cells was higher than in osteoblasts (Fig. 7F). Uncropped blots are available in supplementary (Fig. S1). This evidence concerns the gene TRIM8 and osteosarcoma.