The cytokines VEGF, IL-17, IL-6, IL-8, IL-1Ra, TNF-α, and IL-34 can promote the proliferation of osteosarcoma cells, and TGF-β exerts dual effects on tumors; they inhibit tumor growth in the early stage and promote tumor growth in the late stage. This evidence concerns the gene TGFB1 and osteosarcoma.