To confirm that HSV-1 induced turnover of Nrf2, HEp-2 cells were infected with HSV-1 at different multiplicities of infection (MOI of 0.5, 1, 2 and 5), a dose-dependent reduction in Nrf2 protein levels was observed in infected cells at 24 hpi, supporting the idea that HSV-1 specifically interfered with Nrf2 expression. Here, NFE2L2 is linked to infection.