According to the results of the CCK-8 method to screen the active extracts of S. emarginatum against liver cancer, three doses of 2000 μg/ml, 2500 μg/ml, and 3000 μg/ml of the ethyl acetate extract of S. emarginatum were selected for AnnexinV-PI double staining. The gene discussed is ANXA5; the disease is liver cancer.