In a multivariate analysis of Cox regression for 5-year RFS and 5-year OS (Tables 2 and 3), the effect of a high number of NLN (≥27) in right-side colon cancer was independent of age, sex, CEA level, NLR, subgroups of stage III colon cancer, histology type and grade, and implementation of adjuvant chemotherapy after curative resection (RFS, hazard ratio [HR]: 0.613, 95% confidence interval [CI]: 0.463–0.812, P = 0.001; OS, HR: 0.626, 95% CI: 0.487–0.804, P <  0.001). This evidence concerns the gene CEACAM5 and malignant colon neoplasm.