Our experimental results also demonstrated that overexpressing ISL1 or KDM6B significantly reduced the weight of mice (Additional file 2: Fig. S2F), the degree of liver steatosis (Fig. 6C; Additional file 3: Fig. S3I), as well as the contents of TG and TC (Fig. 6D, E), and after overexpression of ISL1 and inhibition of SNAI1, or overexpression of KDM6B and inhibition of SNAI1, food intake showed no significant difference (Additional file 2: Fig. S2B), and the subcutaneous fat weight was enhanced (Additional file 2: Fig. S2C). The gene discussed is ISL1; the disease is fatty liver disease.