The enhanced TGEV infection was confirmed by TGEV nucleocapsid (N) protein indirect immunofluorescence (IFA) (Fig. 3D), which was in line with the result of transient overexpression of GSDMD, i.e., that the replication of TGEV was significantly inhibited in ST cells transfected with GSDMD vector compared with mock vector control as measured by the quantification of viral RNA, virus titration, and N protein IFA (Fig. 3E to G, respectively). The gene discussed is GSDMD; the disease is infection.