INS and Glucose intolerance: In high-fat-fed (60% fat kcal) C57BL/6 mice, ursolic acid supplementation (0.5 g/kg of HFD) for 12 weeks led to complete normalization of HF diet-induced glucose intolerance and prevented a high-fat diet-induced loss in insulin content, as assessed by immunofluorescence staining of pancreatic tissue sections [188].