In 2020, Gurbatri et al. (2020) used the same synchronized lysis circuit to generate engineered E. coli Nissle 1917 carrying the PD-L1 nanobody (SLC:PD-L1nb) or CTLA-4 nanobody (SLC:CTLA-4nb) in a mouse A20 tumor model. Here, CTLA4 is linked to neoplasm.