USP25 and alkaline phosphatase measurement: Similarly, as shown in Figure 5B and Supplementary Figures 3I–3L, in cerulein-induced AP mice, the level of p-STAT3 protein in USP25−/− AP mice was significantly lower than that in USP25WT AP group, but ZO-1 in USP25−/− AP mice was significantly higher than in USP25WT AP mice.