It was demonstrated that knocking-down HIF-2α in MDA-MB-231 breast cancer cells significantly decreased the expression of glycolysis-related gene products, such as HK2, LDHA, SLC2A1 and PDK1 (Figure 4A), consisting with the suppression of mRNA levels of glycolysis- and hypoxia-related genes (Supplementary Figure S5). Here, PDK1 is linked to breast cancer.