Therefore, we tested the effect of IFN-γ on the expression of the bradyzoite-specific gene BAG1 or the tachyzoite-specific gene SAG1 by using the T. gondii Type II ME49 strain in the following human brain cell lines: astrocytoma cell line (A172), glioblastoma cell line (U-251 MG), and neuroblastoma cell line (IMR-32) (Figures 1A, B). The gene discussed is IFNG; the disease is astrocytoma (excluding glioblastoma).