In conclusion, our pan-cancer EMT analysis of 22 cancer types in TCGA dataset shows that the distinctive features of the EMT-high (mesenchymal) tumors are: (i) the enrichment of TAMs, (ii) overexpression of immune checkpoint molecules, and (iii) overexpression of immune inhibitory cytokines TGFB1 and IL10. The role of TIME in anticancer immunity and immune checkpoint blockade failure is well recognized (47, 48). Here, TGFB1 is linked to cancer.