After detecting the expression of calpain-1 protein in rat myocardial tissue and the calpain activity, the results showed that compared with the control group, the expression of calpain-1 in the myocardial tissue of rats in the T2DM and DAPA groups was higher (Figure 4(a)), and the activity of calpain was evidently higher (Figure 4(b)). This evidence concerns the gene CAPN1 and type 2 diabetes mellitus.