In addition, Western blot experiment indicated that the protein expression levels of calpain-1, p-IκBα/IκBα, and p65 (nucleus) were markedly decreased while the protein expression level of p65 (cytoplasm) was significantly increased in the myocardial tissue of rats in the DAPA group compared with the T2DM group. This evidence concerns the gene CAPN1 and type 2 diabetes mellitus.