In the hG93A SOD1 mouse model, which inherited amyotrophic lateral sclerosis (ALS), EA at ST36 decreased Iba1 protein expression, the number of Iba1-positive cells, and the number of TNF-α positive cells in both the lumbar spinal cord and brain stem and decreased phospho-p38 protein and active caspase-3 expression in the lumbar spinal cord. Here, TNF is linked to amyotrophic lateral sclerosis.