From the co-expression analysis, nine DEE/NDD genes (SYNGAP1, SYN1, IQSEC2, DNM1, PACS1, STXBP1, GRIN1, STX1B, and KIF1A) were highly correlated with MAST3. Known NDD and/or EE genes, such as IQSEC2, DNM1, and STX1B, have all been proved to play a role in vital synaptic functions, such as synaptic vesicle recycling, excitatory synaptic transmission, and calcium-dependent synaptic vesicle release in neurodevelopment (Smirnova et al., 1996; Boumil et al., 2010; Brant et al., 2021). The gene discussed is IQSEC2; the disease is Neurodevelopmental delay.