Univariate regression analysis illustrated that tumor size, vascular invasion, nerve invasion, TNM stage, lymphatic metastasis, distant metastasis, and METTL3 expression were associated with OS in 96 ICC patients, and Multivariate regression analysis demonstrated that METTL3 expression was an independent predictor for overall survival in patients with ICC (HR = 2.105, 95% CI [1.246–3.557] (Supplementary Table 2 and Fig. 1I)). This evidence concerns the gene METTL3 and neoplasm.