Accordingly, we performed RNA-Seq-based transcriptome analysis on 5 and 40-day-old adult fly head homogenate prepared from VRQ ovine PrP Drosophila and the control 51D fly line after larval exposure to either scrapie-infected or control prion-free sheep brain homogenate (Thackray et al. 2020). This evidence concerns the gene PRNP and scrapie.