As shown in Figure 2B and Figure S2A (Supporting Information), PYK2 ablation in BC cells (two independent clones of two sgRNAs; KO2, Figure 2B, and KO12, Figure S2A, Supporting Information) also reduced tumor growth by ≈40%, and the excised tumors displayed similar H&E staining of less condensed tissue (Figure S2B, Supporting Information). The gene discussed is PTK2B; the disease is breast cancer.