In this study, we applied genetic approaches to ablate the PYK2 gene (PTK2B) either in breast cancer cells using the CRISPR/Cas9 technology, in the TME (total knockout mice), or selectively in macrophages (Cx3cr1‐Cretg/wt/PYK2f/f mice). The gene discussed is PTK2B; the disease is breast carcinoma.