We examined both mouse and human breast cancer cell lines; the mouse system included both WT and PYK2 KO BC EO771 cells and the murine Raw264.7 macrophage line, while the human system included several TNBC cell lines (BT549, MDA‐MB‐231, Hs578T; WT and PYK2 knockdown (KD)) and the human monocytic cell line THP‐1.[33] As shown in Figure3A,B, depletion of PYK2 in BC cells significantly attenuated chemotaxis of macrophages toward tumor cells, suggesting that PYK2 depletion modulates the secretome of cancer cells and consequently macrophage attraction. This evidence concerns the gene PTK2B and breast carcinoma.