The influence of PYK2 on CCL2 transcription (Figure 3G–I), led us to examine whether PYK2 regulates CCL2 transcription via Notch signaling, as previous studies showed that Notch1 regulates IL‐1β and CCL2 expression in mammary carcinoma and consequently modulates macrophage recruitment.[39] We, therefore, examined the level of Notch1 protein in WT and PYK2‐depleted human TNBC cell lines (Figure 4A) or murine EO771 cells (Figure 4B; Figure S4A, Supporting Information). Here, IL1B is linked to breast carcinoma.