To evaluate the effects of PYK2 on the macrophage phenotype in the TME, we used fluorescence‐activated cell sorting to isolate TAMs from size‐matched breast tumors in WT and PYK2 KO background (Figure S7A,B, Supporting Information), and analyzed mRNA expression of several pro‐tumorigenic polarization markers (Figure 7K). This evidence concerns the gene PTK2B and breast neoplasm.