Furthermore, we also found DMS in a number of hypomethylated tumor‐promoting gene (including uPA, HPA, TFF3, MAT2A, etc.)and frequently methylated TSGs (DLC1, CHD5, IGFBP2, ADAM23, LRRC4, LASS2, SCAI, HOXB13, CSRNP1, RASSF1A, RB1, GSTP1, NQO1, PROX1, NORE1B, RIZ1, RELN, etc.)which identified in primary human HCC,4, 11 either in Hep3B cell or PLC/PRF/5 with ZNF191 knockdown. Here, CERS2 is linked to hepatocellular carcinoma.