Furthermore, we also found DMS in a number of hypomethylated tumor‐promoting gene (including uPA, HPA, TFF3, MAT2A, etc.)and frequently methylated TSGs (DLC1, CHD5, IGFBP2, ADAM23, LRRC4, LASS2, SCAI, HOXB13, CSRNP1, RASSF1A, RB1, GSTP1, NQO1, PROX1, NORE1B, RIZ1, RELN, etc.)which identified in primary human HCC,4, 11 either in Hep3B cell or PLC/PRF/5 with ZNF191 knockdown. This evidence concerns the gene LRRC4 and neoplasm.