Mucus is an essential component of both the physiological and pathological processes in the airway.38, 39 Airway mucus plugging can affect asthma progression and aggravation, leading to death.40, 41, 42 Under asthmatic conditions, excessive production of MUC5AC contributes to mucous plugs and airflow obstruction.43, 44 In this study, we detected MUC5AC expression, which was significantly elevated in the lungs of HDM‐treated AAV5‐CTNNAL1‐RNAi mice. This evidence concerns the gene MUC5AC and asthma.