Moreover, results of a FISH analysis showed that hsa_Circ_0020256 was mainly expressed in proliferating cells that were positive for Ki67 immunostaining, and the levels of Circ_0020256 in proliferating cells were significantly higher in the clinical tumor tissues of CCA patients than in their paracancerous tissues (Fig. 8F). The gene discussed is MKI67; the disease is neoplasm.