In accordance with these premises, here we demonstrated that the pharmacological interaction of TF specifically with INTB1 ameliorated obesity, and the use of known outside-in blockers such as HMB1, RGDS, CIL and EPT [39, 40, 54] blocked the effect of TF while they were not able to reduce TG content by themselves. This evidence concerns the gene TF and obesity due to melanocortin 4 receptor deficiency.