In summary, in this study, we focused on the ZFAS1 regulatory role and mechanism in the progression of DN and found that ZFAS1 regulates the proliferation, oxidative stress, fibrosis, and inflammation of HG-induced DN by downregulating miR-588 expression and upregulating ROCK1 mRNA and protein expression. Here, ZFAS1 is linked to liver dysplastic nodule.