The further functional analysis showed that the inhibitor of Wnt/β-catenin (IWR-1-endo) could almost block the CTx-resistance induced by miR-199b-3p, verifying our finding that miR-199b-3p contributes to acquired resistance to CTx in CRC through regulating Wnt/β-catenin signaling. The gene discussed is SLC7A6OS; the disease is colorectal carcinoma.