Afterward, we found that the treatment of IWR-1-endo could significantly reverse the CTx resistance promoted by miR-199b-3p, indicating that miR-199b-3p contributes to acquired resistance to CTx in CRC cells via the Wnt/β-catenin signaling pathway (Fig. 5B–E, Additional file 2: Fig. S2C). Here, CYP27A1 is linked to colorectal carcinoma.