However, in our study, positive SGLT-1 staining was found predominantly in the cytoplasm and some membranes, while little staining was observed in the nucleus of malignant duct cells of PDAC and barely in adjacent normal tissue (mean score of tumour vs. normal tissue 5.273 vs. 0.760, P < 0.0001, Fig. 1D), consistent with the conclusion of the study by Casneuf et al. [41]. This evidence concerns the gene SLC5A1 and neoplasm.