CD40LG and Lyme disease: To aid earlier detection of LB and to comply with the National Institute for Clinical Excellence (NICE) guidelines for Lyme disease published in 2018 [2], immunoblot for the detection of IgM antibodies to B. burgdorferi was introduced in 2018, followed by the introduction of IgM (and IgG) chemiluminescent immunoassay (CLIA) in 2020.