Furthermore, we found that submucosal injection of the DNA encoding mutated E6 and mutated E7 in combination with DNA encoding AKT, c-Myc, and SB100 in the submucosa of the oral/pharyngeal cavity followed by EP induced a spontaneous squamous cell carcinoma in HLA-A2 (AAD) mice. The gene discussed is AKT1; the disease is squamous cell carcinoma.