Among the differentially expressed secreted proteins between the two treatments, we selected SCUBE3 for further analysis, as it has been shown to regulate early lung cancer angiogenesis and metastasis (Figure 4e).[26] In addition, we found DEPDC1B was predominantly localized in the cytoplasm of melanoma specimens (Figure 1e), indicating it does not have a role in regulating gene transcription. Here, SCUBE3 is linked to lung cancer.