Previous studies using a single shRNA demonstrated that DEPDC1B is required for melanoma proliferation and tumorigenicity.[20] To consolidate the findings, we used two different shRNA lentiviral constructs to stably silence DEPDC1B transcripts (KD1, KD2) in BRAF‐mutated WM266‐4, A2058, and SK‐MEL‐28 melanoma cell lines and obtained similar outcomes with a marked reduction of proliferation, colony formation and subcutaneous tumor growth compared with controls (Figure S1a–d, g, Supporting Information). The gene discussed is DEPDC1B; the disease is melanoma.