SCUBE3 and melanoma: To further investigate whether SCUBE3 secreted from melanoma cells contributes to angiogenesis, we designed two shRNAs to deplete SCUBE3 mRNA (SCUBE3 KD1 and KD2) and proteins (Figure5a,b), and generated lentiviral vector‐mediated SCUBE3 overexpression (SCUBE3 OE) in both WM266‐4 and A2058 cells (Figure 5c,d).