It is possible that distinct epigenetic regulation at the DEPDC1B promoter and availability of SOX10 cofactors in different cell lines could modulate SOX10 binding capacities to different motifs as evidenced by previous studies in other cellular contexts.[29, 30, 31] Notably, the ChIP signals of SOX10 binding to DEPDC1B promoter in both cell lines were much weaker than that to MIA positive control promoter which has been shown to bind SOX10 in melanoma cell lines[32] (Figure 3c), implying distinct binding capacities of SOX10 on different target gene promoters. This evidence concerns the gene SOX10 and melanoma.