We focused on RHOA and RAC1, whose activities have been shown to be regulated by DEPDC1B in different cellular contexts.[11, 34, 35] We used RHOA and RAC1 activation assays to examine the impact of DEPDC1B KD and OE on the levels of the active forms of RHOA‐GTP and RAC1‐GTP in melanoma cells, respectively. The gene discussed is RAC1; the disease is melanoma.