We used SPPV_56 gene in this study, as it encodes an ortholog of the well-studied L1R protein of the vaccinia virus,which was used as a live smallpox vaccine in the 20th century.The L1R is a membrane protein of the infectious intracellularmature virion (IMV) and is required for the virus to enter thecell (Bisht et al., 2008). The gene discussed is ACKR5; the disease is smallpox.