NPY5R and metastatic malignant neoplasm in the lymph nodes: In addition, tumors with high NPY5R methylation had significantly lymph node metastasis (N3 vs. N1, p < 0.05), depth of invasion (T3 vs. T1, p < 0.01), and higher TNM stage (III vs. I/II, p < 0.05) (Supplementary Figures S2J–L).