GSDME and infection: We found that, 24 h after AH/1 infection, the PI positive-stained populations of A549-vector control cells and A549-GSDME/− cells were 56.3% and 9.7%, respectively (Fig. 5E), and that the PI positive-stained populations of Raw-vector control cells and Raw-GSDME/+ cells were 26.4% and 67.5%, respectively (Fig. 5F).