To validate the most significant genes identified across both models as conferring resistance to rTRAIL, we used synthetic crRNA to knock out expression of FADD, BID, CASP8, and TNFRSF10B (TRAIL-R2) in the rTRAIL-sensitive MSTO-211H mesothelioma cell line stably expressing Cas9 (Fig. 1C). Here, CASP8 is linked to mesothelioma.